Stable cycling and low-temperature operation utilizing amorphous carbon-coated graphite anodes for lithium-ion batteries.

With the continuous expansion of the lithium-ion battery market, addressing the critical issues of stable cycling and low-temperature operation of lithium-ion batteries (LIBs) has become an urgent necessity. The high anisotropy and poor kinetics of pristine graphite in LIBs contribute to the formation of precipitated lithium dendrites, especially during rapid charging or low-temperature operation. In this study, we design a graphite coated with amorphous carbon (GC) through the Chemical Vapor Deposition (CVD) method. The coated carbon layer at the graphite interface exhibits enhanced reaction kinetics and expanded lithium-ion diffusion pathways, thereby reduction in polarization effectively alleviates the risk of lithium precipitation during rapid charging and low-temperature operation. The pouch cell incorporating GC‖LiCoO2 exhibits exceptional durability, retaining 87% of its capacity even after 1200 cycles at a high charge/discharge rate of 5C/5C. Remarkably, at -20 °C, the GC-2 maintains a specific capacity of 163 mA h g-1 at 0.5C, higher than that of pristine graphite (65 mA h g-1). Even at -40 °C, the GC-2‖LiCoO2 pouch cell still shows excellent capacity retention. This design realizes the practical application of graphite anode in extreme environments, and have a promising prospect of application.

Discovery of two novel bioactive algicidal substances from Brevibacillus sp. via metabolomics profiling and back-validation.

Identifying potent bacterial algicidal agents is essential for the development of effective, safe, and economically viable algaecides. Challenges in isolating and purifying these substances from complex secretions have impeded progress in this field. Metabolomics profiling, an efficient strategy for identifying metabolites, was pioneered in identifying bacterial algicidal substances in this study. Extracellular secretions from different generations of the algicidal bacterium Brevibacillus sp. were isolated for comprehensive analysis. Specifically, a higher algicidal efficacy was observed in the secretion from Generation 3 (G3) of Brevibacillus sp. compared to Generation 1 (G1). Subsequent metabolomics profiling comparing G3 and 1 revealed 83 significantly up-regulated metabolites, of which 9 were identified as potential algicidal candidates. Back-validation highlighted the potency of 4-acetamidobutanoic acid (4-ABC) and 8-hydroxyquinoline (8-HQL), which exhibited robust algicidal activity with 3d-EC50 values of 6.40 mg/L and 92.90 µg/L, respectively. These substances disrupted photosynthetic activity in M. aeruginosa by ceasing electron transfer in PSⅡ, like the impact exerted by Brevibacillus sp. secretion. These findings confirmed that 4-ABC and 8-HQL were the main algicidal components derived from Brevibacillus sp.. Thus, this study presents a streamlined strategy for identifying bacterial algicidal substances and unveils two novel and highly active algicidal substances. ENVIRONMENTAL IMPLICATION: Harmful cyanobacterial blooms (HCBs) pose significant environmental problems and health effects to humans and other organisms. The increasing frequency of HCBs has emerged as a pressing global concern. Bacterial-derived algicidal substances are expected to serve as effective, safe, and economically viable algaecides against HCBs. This study presents a streamlined strategy for identifying bacterial algicidal substances and unveils two novel substances (4-ABC and 8-HQL). These two substances demonstrate remarkable algicidal activity and disrupt the photosynthetic system in M. aeruginosa. They hold potential as prospective algaecides for addressing HCBs.

Generation of a DMD loss-of-function mutant human embryonic stem cell lines by CRISPR base editing.

Duchenne muscular dystrophy (DMD) is a fatal X-linked recessive disorder, which is caused mostly by frame-disrupting, out-of-frame variation in the dystrophin (DMD) gene. Loss-of- function mutations are the most common type of mutation in DMD, accounting for approximately 60-90% of all DMD variations. In this study, we used adenine base editing to generate a human embryonic stem cell line with splice-site mutations to mimic exon deletion variants in clinical Duchenne muscular dystrophy patients. This cell line has differentiation potential and a normal karyotypic.

Harmony in detoxification: Microalgae unleashing the potential of lignocellulosic pretreatment wastewater for resource utilization.

Lignocellulosic biomass is a pivotal renewable resource in biorefinery process, requiring pretreatment, primarily chemical pretreatment, for effective depolymerization and subsequent transformation. This process yields solid residue for saccharification and lignocellulosic pretreatment wastewater (LPW), which comprises sugars and inhibitors such as phenols and furans. This study explored the microalgal capacity to treat LPW, focusing on two key hydrolysate inhibitors: furfural and vanillin, which impact the growth of six green microalgae. Chlorella sorokiniana exhibited higher tolerance to furfural and vanillin. However, both inhibitors hindered the growth of C. sorokiniana and disrupted algal photosynthetic system, with vanillin displaying superior inhibition. A synergistic inhibitory effect (Q < 0.85) was observed with furfural and vanillin on algal growth. Furfural transformation to low-toxic furfuryl alcohol was rapid, yet the addition of vanillin hindered this process. Vanillin stimulated carbohydrate accumulation, with 50.48 % observed in the 0.1 g/L furfural + 0.1 g/L vanillin group. Additionally, vanillin enhanced the accumulation of C16: 0 and C18: 2, reaching 21.71 % and 40.36 %, respectively, with 0.1 g/L vanillin. This study proposed a microalgae-based detoxification and resource utilization approach for LPW, enhancing the comprehensive utilization of lignocellulosic components. The observed biomass modifications also suggested potential applications for biofuel production, contributing to the evolving landscape of sustainable biorefinery processes.

Enhanced Photoelectric Properties of CsPbBr3 by SiO2 and TiO2 Bilayer Heterostructures.

CsPbBr3/SiO2 heterostructures were synthesized by the hydrolysis reaction of a mixture of CsPbBr3 nanocrystals (NCs) and (3-aminopropyl)triethoxysilane (APTS) in air. Compared with CsPbBr3 NCs, the CsPbBr3/SiO2 heterostructures exhibit stronger photoluminescence (PL) intensity, longer lifetime of PL (∼40.5 ns), and higher PL-quantum yield (PLQY, ∼86%). The carrier dynamics of CsPbBr3/SiO2 was detected by the transient absorption (TA) spectrum. The experimental results show that SiO2 passivates the surface traps of CsPbBr3 NCs and enhances the PL intensity. However, photoelectrochemical impedance spectra (PEIS) demonstrate that the impedance of CsPbBr3/SiO2 is higher than that of CsPbBr3 NCs, which reduces carrier transport and extraction. Because the application of CsPbBr3/SiO2 in optoelectronics is limited, CsPbBr3/SiO2/TiO2 heterostructures were synthesized by the further reaction of tetrabutyl titanate (TBT). The TiO2 coating can reduce the impedance of the CsPbBr3/SiO2. Importantly, ∼68% of the PL intensity of CsPbBr3/SiO2 is retained. Compared with CsPbBr3/SiO2 and CsPbBr3 NCs, the CsPbBr3/SiO2/TiO2 demonstrates faster carrier transport (κct = 2.4 × 109 s-1) and higher photocurrent density (J = 76 nA cm-2). In addition, CsPbBr3/SiO2/TiO2 shows good stability under (ultraviolet) UV irradiation, along with water stability and thermal stability. Therefore, the double protection approach can enhance the stability of CsPbBr3 NCs and tune the optoelectronic properties of CsPbBr3 NCs.

Treatment of mixed wastewater by vertical rotating microalgae-bacteria symbiotic biofilm reactor.

A novel vertical rotating microalgae-bacteria symbiotic biofilm reactor was built to treat the mixed wastewater containing municipal and soybean soaking wastewater. The reactor was operated in both sequential batch and semi-continuous modes. Under the sequential batch operation mode, the maximum removal rates for Chemical Oxygen Demand (COD), Total Nitrogen (TN), Total Phosphorus (TP), and Ammonia Nitrogen (NH4+-N) of the mixed wastewater were 95.6 %, 96.1 %, 97.6 %, and 100 %, respectively. During the semi-continuous operation, the water discharge indices decreased gradually and eventually stabilized. At stabilization, the removal rates of COD, TN, and NH4+-N achieved 98 %, 95 %, and 99.9 %, respectively. The maximum biomass productivity of the biofilm was 2.69 g·m-2·d-1. Additionally, the carbohydrate, protein and lipid comprised approximately 22 %, 51 % and 10 % of the dry weight of Chlorella. This study demonstrates the great potential of the microalgae-bacteria symbiotic biofilm system to treat food and domestic wastewater while harvesting microalgal biomass.

Fully bio-based epoxy resins from lignin and epoxidized soybean oil: Rigid-flexible, tunable properties and high lignin content.

The application of epoxidized soybean oil (ESO) in thermosetting polymers is impeded by its unsatisfactory thermomechanical properties. Here, in order to address the limitation, technical lignin was modified by tung oil anhydride and then used as the hardener to compensate for the inherent flexibility defects of ESO thermosets (TLs). As the lignin content increased, a notable improvement in the activation energy of TLs was observed, attributed to the restraining effect of lignin's rigid structure on segmental relaxation. Concurrently, the tensile strength of TLs increased from 2.8 MPa to 34.0 MPa, concomitant with a decrease in elongation at break from 32.9 % to 8.0 %. Comparative analysis with TL-0 (devoid of lignin) demonstrated substantial enhancements in glass transition temperature, shape fixation ratio, and shape recovery ratio for TL-50 (comprising 50 wt% of lignin), elevating from 16.9 °C, 89.1 %, and 89.5 % to 118.6 °C, 94.0 %, and 99.3 %, respectively. These results unequivocally highlight the favorable dynamic mechanical and shape memory properties conferred upon TLs by lignin addition. While the introduction of lignin adversely affected thermal stability, a notable improvement in char yield (800 °C) was observed. Collectively, these findings underscore the potential of technical lignin as a promising bio-based curing agent for ESO.

An interpretable artificial intelligence framework for designing synthetic lethality-based anti-cancer combination therapies.

INTRODUCTION: Synthetic lethality (SL) provides an opportunity to leverage different genetic interactions when designing synergistic combination therapies. To further explore SL-based combination therapies for cancer treatment, it is important to identify and mechanistically characterize more SL interactions. Artificial intelligence (AI) methods have recently been proposed for SL prediction, but the results of these models are often not interpretable such that deriving the underlying mechanism can be challenging. OBJECTIVES: This study aims to develop an interpretable AI framework for SL prediction and subsequently utilize it to design SL-based synergistic combination therapies. METHODS: We propose a knowledge and data dual-driven AI framework for SL prediction (KDDSL). Specifically, we use gene knowledge related to the SL mechanism to guide the construction of the model and develop a method to identify the most relevant gene knowledge for the predicted results. RESULTS: Experimental and literature-based validation confirmed a good balance between predictive and interpretable ability when using KDDSL. Moreover, we demonstrated that KDDSL could help to discover promising drug combinations and clarify associated biological processes, such as the combination of MDM2 and CDK9 inhibitors, which exhibited significant anti-cancer effects in vitro and in vivo. CONCLUSION: These data underscore the potential of KDDSL to guide SL-based combination therapy design. There is a need for biomedicine-focused AI strategies to combine rational biological knowledge with developed models.

Recent advances progress of targeted drugs combined with radiotherapy for advanced non-small cell lung cancer: a review.

Targeted drug therapy plays an important role in the clinical application of non-small cell lung cancer, especially adenocarcinoma. However, for patients with advanced disease, drug resistance after targeted therapy, unclear target, and other reasons that cannot or do not want surgery, the combination of chemotherapy, radiotherapy, immunity, etc. is often used. The synergistic effect of targeted drugs and radiotherapy in non-small cell lung cancer has shown good clinical efficacy. This article reviews the clinical progress of targeted drug therapy combined with radiotherapy in advanced non-small cell lung cancer in recent years, in order to provide new ideas for further clinical research of this treatment mode.

Simultaneous saccharification of hemicellulose and cellulose of corncob in a one-pot system using catalysis of carbon based solid acid from lignosulfonate.

The drive towards sustainable chemistry has inspired the development of active solid acids as catalysts and ionic liquids as solvents for an efficient release of sugars from lignocellulosic biomass for future biorefinery practices. Carbon-based solid acid (SI-C-S-H2O2) prepared from sodium lignosulfonate, a waste of the paper industry, was used with water or ionic liquid to hydrolyze corncob in this study. The effects of various reaction parameters were investigated in different solvent systems. The highest xylose yield of 83.4% and hemicellulose removal rate of 90.6% were obtained in an aqueous system at 130 °C for 14 h. After the pretreatment, cellulase was used for the hydrolysis of residue and the enzymatic digestibility of 92.6% was obtained. Following these two hydrolysis steps in the aqueous systems, the highest yield of total reducing sugar (TRS) was obtained at 88.1%. Further, one-step depolymerization and saccharification of corncob hemicellulose and cellulose to reducing sugars in an IL-water system catalyzed by SI-C-S-H2O2 was conducted at 130 °C for 10 h, with a high TRS yield of 75.1% obtained directly. After recycling five times, the solid acid catalyst still showed a high catalytic activity for sugar yield in different systems, providing a green and effective method for lignocellulose degradation.

Recent advances progress in radiotherapy for breast cancer after breast-conserving surgery: a review.

Adjuvant radiotherapy after breast-conserving surgery has become an integral part of the treatment of breast cancer. In recent years, the development of radiotherapy technology has made great progress in this field, including the comparison of the curative effects of various radiotherapy techniques and the performance of the segmentation times. The choice of radiotherapy technology needs to be co-determined by clinical evidence practice and evaluated for each individual patient to achieve precision radiotherapy. This article discusses the treatment effects of different radiotherapy, techniques, the risk of second cancers and short-range radiation therapy techniques after breast-conserving surgery such as hypo fractionated whole breast irradiation and accelerated partial breast irradiation. The choice of radiotherapy regimen needs to be based on the individual condition of the patient, and the general principle is to focus on the target area and reduce the irradiation of the normal tissues and organs. Short-range radiotherapy and hypofractionated are superior to conventional radiotherapy and are expected to become the mainstream treatment after breast-conserving surgery.

Linking Entrepreneurs' Felt Responsibility to Ventures' Innovation Performance: The Roles of External Learning and Shared Vision.

Introduction: In the aftermath of the COVID-19 pandemic, innovation has become a crucial factor for the success of entrepreneurial ventures in China. However, the mechanisms underlying the promotion of innovation by entrepreneurs are not yet fully understood. Drawing on self-determination theory, this study develops a comprehensive "motivation-behaviors-performance" model to examine the relationship between entrepreneurs' felt responsibility for constructive change and ventures' innovation performance. Methods: Two waves of questionnaire surveys with a sample of 376 entrepreneurs in East China were conducted. Hierarchical regression analysis was performed to test the hypotheses. Results: The empirical findings demonstrate that entrepreneurs' felt responsibility for constructive change is a key driver of innovation performance in ventures. Furthermore, external learning, encompassing technological and market learning, serves as a mediator for the positive link between entrepreneurs' felt responsibility and innovation performance. The study also reveals that entrepreneurs' perception of shared vision moderates the relationships between entrepreneurs' felt responsibility and both technological and market learning, such that the relationships are stronger when the perception of shared vision is higher. Discussion: These results yield important contributions to the entrepreneurial psychology research and self-determination theory. The study also has managerial implications for entrepreneurs aiming to enhance ventures' innovation performance.

[Bioconversion of C1 gases and genetic engineering modification of gas-utilizing microorganisms].

C1 gases including CO, CO2 and CH4, are mainly derived from terrestrial biological activities, industrial waste gas and gasification syngas. Particularly, CO2 and CH4 are two of the most important greenhouse gases contributing to climate change. Bioconversion of C1 gases is not only a promising solution to addressing the problem of waste gases emission, but also a novel route to produce fuels or chemicals. In the past few years, C1-gas-utilizing microorganisms have drawn much attention and a variety of gene-editing technologies have been applied to improve their product yields or to expand product portfolios. This article reviewed the biological characteristics, aerobic or anaerobic metabolic pathways as well as the metabolic products of methanotrophs, autotrophic acetogens, and carboxydotrophic bacteria. In addition, gene-editing technologies (e.g. gene interruption technology using homologous recombination, group Ⅱ intron ClosTron technology, CRISPR/Cas gene editing and phage recombinase-mediated efficient integration of large DNA fragments) and their application in these C1-gas-utilizing microorganisms were also summarized.

Effects of a novel medium-long-medium-type structured lipid synthesized using a two-step enzymatic method on lipid metabolism and obesity protection in experimental mice.

We synthesized a novel, structured lipid containing caprylic acid at its sn-1,3 position and docosahexaenoic acid at its sn-2 position (1,3C-2D-TAG) by using a two-step enzymatic method and then investigated the relationship between the types of fatty acids in the structured lipid and their physiological functions. Furthermore, we compared the effects of similarly structured lipids on postprandial lipid metabolism and obesity protection. The results revealed that the novel structured lipid synthesized using the enzymatic method not only exhibited better physical properties than traditional oils but also had high oxidation stability and crystallization degree. In terms of physiological function, the low-dose 1,3C-2D-TAG group exhibited higher cholesterol and triglyceride levels, lower kidney weight or body weight, and higher serum aspartate aminotransferase and blood urea nitrogen levels than control group, whereas the high-dose 1,3C-2D-TAG group exhibited higher triglyceride levels. Moreover, the medium-dose 1,3C-2D-TAG group had remarkably higher high-density lipoprotein cholesterol levels and lower low-density lipoprotein cholesterol levels than the high-lipid, control, and 1,2,3C-TAG groups; however, the cholesterol and triglyceride levels and kidney and liver functions did not differ significantly among these groups. The study results suggest that 1,3C-2D-TAG can not only facilitate the effective utilization of the energy supplied by medium-chain fatty acids but also help overcome difficulties in the absorption of long-chain polyunsaturated fatty acids.

Defective core-shell NiCo2S4/MnO2 nanocomposites for high performance solid-state hybrid supercapacitors.

The roles of oxygen vacancies to enhance the electrochemical performance were not clearly explained in comprehensive research. Herein, the vertically oriented NiCo2S4/MnO2 core-shell nanocomposites are in situ grown on the nickel foam (NF) surface and activated by oxygen vacancy engineering via a chemical reduction method. The scanning electron microscope (SEM) and transmission electron microscope (TEM) results show the shell-MnO2 is well coated on the core-NiCo2S4. The hierarchical core-shell nanostructures synergistically increase conductivity and provide rich faradaic redox chemical reactions. Moreover, the density functional theory (DFT) calculations further indicate that the electronic properties and structure properties in NiCo2S4/MnO2 electrode of reduction for 60 min (NiCo2S4/MnO2-60) are effectively adjusted by introducing oxygen vacancies. Impressively, the NiCo2S4/MnO2-60 electrode delivers substantially appreciable areal capacity of 2.13 mAh·cm-2 couple with superior rate capability. The as-prepared high-performance electrode material can assemble into solid-state hybrid supercapacitor. The fabricated NiCo2S4/MnO2-60//AC device exhibits an exceptional energy density of 43.16 Wh·kg-1 at a power density of 384.21 W·kg-1 and satisfactory cyclic stability of 92.1 % at current density of 10 mA·cm-2 after 10,000 cycles. In general, the work demonstrates the significance of NiCo2S4/MnO2-60 as a highly redox active electrode material for future practical application in supercapacitors.

Applications-oriented algicidal efficacy research and in-depth mechanism of a novel strain Brevibacillus sp. on Microcystis aeruginosa.

The utilization of algicidal bacteria for the control of harmful algal blooms (HABs) is a promising technology for ecological remediation. In our most recent publication, a novel strain of Brevibacillus sp. was isolated and proved to have significant algicidal activity and stability against Microcystis aeruginosa. In order to verify the algicidal effect of the strain in the practical application scenario, the algicidal efficacy of Brevibacillus sp. under conditions close to water in the environment was investigated. Results indicated that the algicidal threshold of Brevibacillus sp. culture was 3‰ inoculation concentration, and the removal rate of M. aeruginosa reached 100%. The process of Chl-a degradation followed a first-order kinetic model, which could be used to predict the degradation effect of M. aeruginosa in practical applications. Additionally, the inoculation of Brevibacillus sp. culture introduced additional nutrients, some of which remained in the water. Furthermore, the algicidal substances demonstrated good sustainability, with a removal rate of up to 78.53% at 144 h after three repeated uses. At 12 h, the algicidal substances caused a 78.65% increase in malondialdehyde (MDA) content in M. aeruginosa compared to the control group, thereby triggering the antioxidant system of M. aeruginosa. Moreover, algal cell fragments were observed to aggregate. This study provides a promising direction for treating cyanobacterial blooms using algicidal bacteria in practical applications.

Design and prototype test of a high-sensitivity reentrant-cavity based Schottky pickup.

Schottky diagnostics is an important measure for the debunched beam during the injection phase of the Xi'an Proton Application Facility (XiPAF). The existing capacitive Schottky pickup has a relatively low sensitivity and a poor signal-to-noise ratio for the low-intensity beam. A resonant Schottky pickup based on the principle of a reentrant cavity is proposed. The effects of cavity geometric parameters on cavity properties are systematically studied. A prototype was built and tested to validate the simulation results. The prototype has a resonance frequency of 24.23 MHz, a Q value of 635, and a shunt impedance of 19.75 kΩ. The resonant Schottky pickup has the capability to detect as few as 2.3 × 106 protons with an energy of 7 MeV and a momentum spread of about 1% at the injection phase of XiPAF. The sensitivity is two orders of magnitude higher than the existing capacitive pickup.

Effect of stent treatment on hemodynamics in iliac vein compression syndrome with collateral vein.

BACKGROUND: Iliac vein compression syndrome (IVCS) leads to blood flow obstruction in the lower extremities and is usually treated with stents, but stenting may worsen the hemodynamics and increase the risk of thrombosis in the iliac vein. The present work evaluates the advantages and disadvantages of the stent on IVCS with a collateral vein. METHODS: The computational fluid dynamics method is adopted to analyze the preoperative and postoperative flow fields in a typical IVCS. The geometric models of the iliac vein are constructed from medical imaging data. The porous model is used to simulate the flow obstruction in IVCS. RESULTS: The preoperative and postoperative hemodynamic characteristics in the iliac vein are obtained, e.g., the pressure gradient at two ends of the compressive region and the wall shear stress. It is found that the stenting restores the blood flow in the left iliac vein. CONCLUSION: Impacts of the stent are classified into short-term and long-term effects. The short-term effects are beneficial in relieving IVCS, i.e., shortening the blood stasis and reducing the pressure gradient. The long-term effects increase the risk of thrombosis in the stent, i.e., enlarging wall shear stress due to a large corner and a diameter constriction in the distal vessel, and suggests the need to develop a venous stent for IVCS.

Optimum capture size of Trichiurus japonicus in the East China Sea.

Trichiurus japonicus is an economically valuable species in the East China Sea, whose allowable capture size varies with fishing effort in different years. To clarify the optimum capture size for T. japonicus in the East China Sea, we collected samples and data from T. japonicus targeting fishing gears such as trawls, canvas spreader stow nets and longlines from 2016 to 2020. We estimated growth and mortality parameters using the FiSAT II software, and calculated the size limit standards for capture. The results showed that the inflection point of anal length, the critical anal length and the anal length of one-year-old T. japonicus was 382.84, 397.12, and 216.05 mm, respectively. The anal length of maturity was 230.38 mm, and the minimum capture size (anal length) was 219.23 mm. Based on the yield per recruitment analysis using Beverton-Holt model, the current fishery reference points were under overfishing condition (tc=0.38 a, F=2.11), and the suggested optimum capture size was 364.64 mm. There would be a sharp decline of T. japonicus catches in the East China Sea if the suggested minimum capture size was substantially higher, which might be non-practical for local fishermen. Therefore, we suggested 220 mm as the capture size limit, which was close to the anal length of one-year-old fish and the anal length of maturity.

Advances in automatic delineation of target volume and cardiac substructure in breast cancer radiotherapy (Review).

Postoperative adjuvant radiotherapy plays an important role in the treatment of patients with breast cancer. With the continuous development of radiotherapeutic technologies, the requirements for radiotherapeutic accuracy are increasingly high. The accuracy of target volume and organ at risk delineation significantly affects the effect of radiotherapy. Automatic delineation software has been continuously developed for the automatic delineation of target areas and organs at risk. Automatic segmentation based on an atlas and deep learning is a hot topic in current clinical research. Automatic delineation can not only reduce the workload and delineation times, but also establish a uniform delineation standard and reduce inter-observer and intra-observer differences. In patients with breast cancer, especially in patients who undergo left breast radiotherapy, the protection of the heart is particularly important. Treating the whole heart as an organ at risk cannot meet the clinical needs, and it is necessary to limit the dose to specific cardiac substructures. The present review discusses the importance of automatic delineation of target volume and cardiac substructure in radiotherapy for patients with breast cancer.